Rapid separation and simultaneous quantitative determination of 13 constituents in Psoraleae Fructus by a single marker using high‐performance liquid chromatography with diode array detection

Psoraleae Fructus is one of the most popular traditional Chinese medicines. Coumarins, flavonoids, and meroterpenes are the main contributors to the biological activity of Psoraleae Fructus. In this study, a new method for the quality control of Psoraleae Fructus was developed, through the quantitative analysis of multicomponents by single marker with diode array detector. Thirteen components, including psoralenoside, isopsoralenoside, psoralen, isopsoralen, psoralidin, neobavaisoflavone, bavachin, corylin, isobavachalcone, corylifol A, bavachinin, bavachalcone, and bakuchiol were rapidly separated and identified within 12 min by the newly developed method. The feasibility and reliability of this method were corroborated. The method was also compared to the external standard method and detection by corona charged aerosol detector. The results of percent difference (%) and cos (θ) have shown that there were no significant differences observed between the quantitative analysis of multicomponents by single marker and external standard method analyses; psoralen and isopsoralen were undetectable with the corona charged aerosol detector due to their but the sensitivity for all the compounds except bakuchiol detected by corona charged aerosol detector are higher than those obtained by diode array detector. In addition, the newly method developed was applied to the quality evaluation of Chinese patent medicines containing Psoraleae Fructus.     

Directly‐coupled‐column ultra‐fast liquid chromatography with diode array detection method for the rapid quantification of allergenic disperse dyes in water preconcentrated by magnetic solid‐phase extraction

A directly‐coupled‐column ultra‐fast liquid chromatography coupled with diode array detection method for the determination of 12 allergenic disperse dyes in river water at sub‐ppb levels has been developed and successfully validated. The analytical method is based on the use of two different reversed‐phased columns connected through a two‐position switching valve. A baseline separation was achieved by proper selection of stationary phases, mobile phases, and the use of a gradient elution in both dimensions. Furthermore, an easy‐to‐handle magnetic solid‐phase extraction procedure was developed for the preconcentration of 12 allergenic disperse dyes from river water. An enrichment factor of 100 times was obtained. The results showed excellent performance in terms of trueness (76.8–99.0%), precision (intraday: 2.2–8.0%, interday: 3.3–8.2%), and sensitivity (limits of determination, 0.027–1.46 μg/L). Twenty real samples collected from the outfalls in the Yaojiang, Yongjiang and Fenghuajiang estuary were analyzed, and three of the studied compounds were found in one collected sample (12.6 μg/L for disperse blue 7, 11.6 μg/L for disperse blue 106, and 0.22 μg/L for disperse blue 3).     

基于向后逐步回归模型的我国农机需求特征及影响因素研究

以2004-2014年数据为样本,以农机总动力为因变量,以农机购置补贴、农机价格、农机作业服务价格、农户人均纯收入、农产品价格、农业劳动力数量和农作物播种总面积为自变量构建了我国农机需求影响因素的回归方程,向后逐步回归结果表明:(1)除农作物播种总面积、农机作业服务价格、农产品价格之外,其余变量对农机总动力的影响均统计上显著;(2)农机购置补贴、农机价格指数、农户人均纯收入及农业劳动力四个变量对农机需求的弹性系数分别为0.027、-0.004、0.457、0.474。最后根据研究结果提出加大与落实农机购置补贴、规范农机价格、培育新型经营主体、加速土地规范流转等政策建议。     

DCE-MRI定量参数对子宫内膜癌的诊断价值及与临床病理因素的相关性

本文选取了子宫内膜癌患者108例作为观察组,另选取子宫内膜良性病变患者105例作为对照组,均行DCE-MRI检查后发现,观察组容量转移常数(K trans)、速率常数(K ep)、血管外细胞外间隙容积比值(V e)高于对照组(P<0.05);K trans、K ep、V e值联合诊断子宫内膜癌的曲线下面积(AUC)为0.841;K trans、K ep、V e值与临床分期、肌层浸润程度呈正相关,与分化程度呈负相关(P<0.05)。本研究结果提示DCE-MRI定量参数对子宫内膜癌具有一定鉴别诊断价值,有助于进一步区分子宫内膜癌患者肌层浸润程度、临床分期、分化程度。     

Lemon Oils Attenuate the Pathogenicity of Pseudomonas aeruginosa by Quorum Sensing Inhibition

The chemical composition of three Citrus limon oils: lemon essential oil (LEO), lemon terpenes (LT) and lemon essence (LE), and their influence in the virulence factors production and motility (swarming and swimming) of two Pseudomonas aeruginosa strains (ATCC 27853 and a multidrug-resistant HT5) were investigated. The main compound, limonene, was also tested in biological assays. Eighty-four compounds, accounting for a relative peak area of 99.23%, 98.58% and 99.64%, were identified by GC/MS. Limonene (59–60%), γ-terpinene (10–11%) and β-pinene (7–15%) were the main compounds. All lemon oils inhibited specific biofilm production and bacterial metabolic activities into biofilm in a dose-dependent manner (20–65%, in the range of 0.1–4 mg mL⁻¹) of both strains. Besides, all samples inhibited about 50% of the elastase activity at 0.1 mg mL⁻¹. Pyocyanin biosynthesis decreases until 64% (0.1–4 mg mL⁻¹) for both strains. Swarming motility of P. aeruginosa ATCC 27853 was completely inhibited by 2 mg mL⁻¹ of lemon oils. Furthermore, a decrease (29–55%, 0.1–4 mg mL⁻¹) in the synthesis of Quorum sensing (QS) signals was observed. The oils showed higher biological activities than limonene. Hence, their ability to control the biofilm of P. aeruginosa and reduce the production of virulence factors regulated by QS makes lemon oils good candidates to be applied as preservatives in the food processing industry.     

阴极微弧电沉积制备Al2O3陶瓷层技术及研究进展

阴极微弧电沉积技术是一种在材料表面通过微弧放电沉积陶瓷层的表面处理技术,利用该技术可以在金属及非金属表面生成耐磨、耐蚀性能优异的陶瓷膜层.本文介绍了阴极微弧电沉积技术的研究现状以及应用阴极微弧电沉积技术制备Al2O3陶瓷层的方法和基本原理,并且对阴极微弧电沉积技术的影响因素进行了总结,阐述了阴极微弧电沉积技术的应用前景和存在的问题.     

聚合物结构对包膜缓释肥缓释性能的影响

研发和使用缓控释肥能有效解决普通肥料利用率低以及由于化肥使用过量而引起的环境问题。聚合物包膜型缓控释肥是缓控释肥中的一种,影响其缓释性能的因素如环境因素、包膜厚度、加工工艺等,前人已研究较多。聚合物结构对于缓释性能的影响也很重要,而这方面研究较少。本文主要讨论了聚合物结晶性、分子亲疏水性、分子链柔顺性、交联等结构因素对缓释性能的影响。深入研究聚合物结构对缓释性能的影响有助于缓控释材料的创新;能为聚合物包膜材料的设计、聚合物缓控释肥的研发及生产提供一定的理论依据。     

A centrally cracked thin circular disk, Part I: 3D Elastic–plastic finite element analysis

A full field solution, based on small deformation, three-dimensional elastic–plastic finite element analysis of the centrally cracked thin disk under mode I loading has been performed. The solution for the stresses under small-scale yielding and lo!cally fully plastic state has been compared with the HRR plane stress solution. At the outside of the 3D zone, within a distance of rσ_o/J=18, HRR dominance is maintained in the presence of a significant amount of compressive stress along the crack flanks. Ahead of this region, the HRR field overestimate the stresses. These results demonstrate a completely reversed state of stress in the near crack front compared to that in the plane strain case. The combined effect of geometry and finite thickness of the specimen on elastic–plastic crack tip stress field has been explored. To the best of our knowledge, such an attempt in the published literature has not been made yet. For the qualitative assessment of the results some of the field parameters have been compared to the available experimental results of K, gives a fair estimate of the crack opening stress near the crack front at a distance of order 10⁻² in. On the basis of this analysis, the Linear Elastic Fracture Mechanics approach has been adopted in analyzing the fatigue crack extension experiments performed in the disk (Part II).     

Interaction of a screw dislocation with an interfacial edge crack in a two-phase piezoelectric material

The interaction of a screw dislocation with an interfacial edge crack in a two-phase piezoelectric medium is investigated. Closed-form solutions of the elastic and electrical fields induced by the screw dislocation are derived using the conformal mapping method in conjunction with the image principle. Based on the electroelastic fields derived, the stress and electric displacement intensity factors, the image force acting on the dislocation are given explicitly. We find that the stress and electric displacement intensity factors depend on the effective electroelastic material constants. In the case where one of two phases is purely elastic, the stress intensity factor and image force are plotted to illustrate the influences of electromechanical coupling effect, the position of the dislocation and the material properties on the interaction mechanism. The project supported by the Doctoral Foundation of Hebei Province (B2003113)     

Stress intensity factors and crack deviation conditions in a brittle anisotropic solid

For arbitrary anisotropy in the linear manifold of singular solutions generating square-root singularities of the crack tip stress, a special basis is introduced that possesses the same properties as in the isotropic case and provides simple integral representations for the attributes of the energy fracture criterion, in particular, the conditions of crack deviation from a straight path. __________ Translated from Prikladnaya Mekhanika i Tekhnicheskaya Fizika, Vol. 46, No. 3, pp. 98–107, May–June, 2005.